Topo ta cloning kit pcr2.1 manual dexterity

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coli. Vector 1 contains everything except the tip (FimH), and Vector 2 contains FimH. Vector 1 is a pCRII-TOPO plasmid with ColE1 (pUC) origin of replication.
I’m using the CloneEZ PCR cloning kit to perform some DNA library construction. In the manual, it recommends using linearized vectors with a concentration of
I’ve cloned my gene into the pCR2.1-TOPO vector and transformed into the TOP10 cells I’m subcloning fragments of yeast genomic DNA into a TOPO vector.